HYDROLYSIS OF STARCH WITH IMMOBILIZED GLUCOAMYLASE - A COMPARISON BETWEEN 2 TYPES OF EXPANDED-BED REACTORS

被引：10

作者：

FREIRE, DMG

SANTANNA, GL

机构：

[1] UNIV FED RIO DE JANEIRO,COPPE,ESCOLA QUIM,POB 68502,BR-21945 RIO DE JANEIRO,BRAZIL

[2] UNIV FED RIO DE JANEIRO,INST QUIM,BR-21945 RIO DE JANEIRO,BRAZIL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 26卷 / 01期

关键词：

enzymatic reactors; Immobilized glucoamylase; immobilized-enzyme bioreactors; starch hydrolysis;

D O I：

10.1007/BF02798390

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucoamylase (E.C.3.2.1.3) covalently immobilized onto chitin particles (dst = 0.37 mm) was examined in two types of continuous bench-scale reactors (180 mL) fed with hydrolyzed manioc starch (15%, w/v): a two-phase reactor (liquid expanded-bed) and a threephase reactor (air expanded-bed). Several conditions of continuous operation were investigated, varying the biocatalyst load (16.7, 37.2, and 54 g/L) into the reactor and the hydraulic residence time. The best results were achieved with the two-phase reactor, which operated continuously for 20 d and showed a decrease of only 6% in conversion (starch to glucose). Conversion levels of 96% were obtained with a hydraulic residence time of about 4 h. A simple mathematical model was able to describe the experimental results of the two types of reactors considering biocatalyst deactivation. © 1990 Humana Press Inc.

引用

页码：23 / 34

页数：12

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