INACTIVATION OF LIPOPROTEIN LIPASE IN BUFFERED SALINE SOLUTIONS

1. 1. The rate of inactivation of lipoprotein lipase (glycerol-ester hydrolase, EC 3.1.1.3) in dilute buffered saline solutions has been investigated, and found to be dependent on the ionic strength of the incubation medium and not upon its molar concentration. 2. 2. Unesterified long-chain fatty acids protect the enzyme from the effects of ions by formation of stabilised enzyme-free fatty acid complexes. 3. 3. Lipoprotein lipase purified from post-heparin plasma, or adipose, heart, lung or diaphragm tissues shows the same first-order kinetics of inactivation in each case, and appears to represent a single enzyme species. 4. 4. Crude enzyme preparations from these tissues are partially stabilised in comparison with the purified enzyme. © 1968.