THE PROMOTER OF THE CHICKEN ALPHA-2(VI) COLLAGEN GENE HAS FEATURES CHARACTERISTIC OF HOUSE-KEEPING GENES AND OF PROTOONCOGENES

被引：38

作者：

KOLLER, E ^{[1
]}

HAYMAN, AR ^{[1
]}

TRUEB, B ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,BIOCHEM LAB 1,CH-8092 ZURICH,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 03期

关键词：

D O I：

10.1093/nar/19.3.485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The promoter of the chicken alpha-2(VI) collagen gene reveals several interesting features characteristic of house-keeping genes and growth control related genes. It does not possess a typical TATAA or CAAT box, but it contains several potential binding sites for transcription factors Sp1 and ETF. The 5' flanking region of the gene forms a typical 'CpG island' where the dinucleotide sequence CpG occurs with high frequency relative to the bulk genome. Consistent with the lack of a TATAA element, the gene contains multiple transcription initiation sites distributed over 75 bp of genomic DNA. A short DNA fragment (207 bp) encompassing all the transcription initiation sites and the entire CpG island shows strong promoter activity when linked to a heterologous reporter gene. The upstream region of the promoter harbours a long homopurine/homopyrimidine element (403 bp) which is sensitive to endonuclease S1. This element might have the ability to adopt an intramolecular hairpin triplex structure and could play a role in the organization of the chromatin at the alpha-2(VI) collagen locus. Our results demonstrate that the structure of the alpha-2(VI) collagen promoter is completely different from that of any other collagen promoter characterized so far.

引用

页码：485 / 491

页数：7

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