IDENTIFICATION OF RASPBERRY CULTIVARS BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

被引：19

作者：

PARENT, JG ^{[1
]}

FORTIN, MG ^{[1
]}

PAGE, D ^{[1
]}

机构：

[1] MCGILL UNIV, FAC SCI AGR & ENVIRONM, Ste Anne De Bellevue H9X 3V9, PQ, CANADA

来源：

CANADIAN JOURNAL OF PLANT SCIENCE | 1993年 / 73卷 / 04期

关键词：

RASPBERRY (RUBUS SPP); RANDOM AMPLIFIED POLYMORPHIC DNA; CULTIVAR IDENTIFICATION;

D O I：

10.4141/cjps93-150

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We developed a fast and simple test to accurately characterize the various cultivars of raspberry of the Quebec certification program. This test, based on random amplified polymorphic DNA (RAPD) analysis, uses minute quantities of plant DNA. Portions of this DNA are multiplied with small 10-nucleotide primers of arbitrary sequences. Analyses were done on DNA from 15 raspberry cultivars amplified with 19 different primers. A combination of three primers was chosen for its ability to differenciate all the cultivars. Test reproducibility was checked with DNA samples from plants collected at various periods of the growth season and from plants of various categories of the certification program (in vitro plantlets, Foundation and Certified plants, commercial plants).

引用

页码：1115 / 1122

页数：8

共 23 条

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