APPLICATION OF ISOTHERMAL TITRATION CALORIMETRY FOR DETECTING LIPID-MEMBRANE SOLUBILIZATION

被引：44

作者：

HEERKLOTZ, H ^{[1
]}

LANTZSCH, G ^{[1
]}

BINDER, H ^{[1
]}

KLOSE, G ^{[1
]}

BLUME, A ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,FACHBEREICH CHEM,D-67653 KAISERSLAUTERN,GERMANY

来源：

CHEMICAL PHYSICS LETTERS | 1995年 / 235卷 / 5-6期

关键词：

D O I：

10.1016/0009-2614(95)00139-U

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Isothermal titration calorimetry is presented to be an excellent method of detecting lipid membrane solubilization upon the addition of detergents. As an example, the titration of the non-ionic detergent C(12)EO(6) to the lipid POPC is studied. The main contribution to the measured titration heat is the detergent's enthalpy difference upon transfer between micelles and membranes. The detergent added as micelles is transferred into membranes up to the saturating detergent per lipid mol/mol ratio, R(sat) = 1.45 +/- 0.07. Beyond R(sat) the membranes are solubilized to micelles. Subsequently, the titration heat changes its sign from endothermic to exothermic. After complete membrane dissolution at R(sol) = 5.0 +/- 0.4 the titration heat vanishes nearly completely.

引用

页码：517 / 520

页数：4

共 15 条

[1] STATES OF AGGREGATION AND PHASE-TRANSFORMATIONS IN MIXTURES OF PHOSPHATIDYLCHOLINE AND OCTYL GLUCOSIDE [J].

ALMOG, S ;

LITMAN, BJ ;

WIMLEY, W ;

COHEN, J ;

WACHTEL, EJ ;

BARENHOLZ, Y ;

BENSHAUL, A ;

LICHTENBERG, D .

BIOCHEMISTRY, 1990, 29 (19) :4582-4592

[2] CALORIMETRIC DETERMINATION OF THE ENTHALPY OF MICELLE FORMATION IN AQUEOUS-MEDIA [J].

BIRDI, KS .

COLLOID AND POLYMER SCIENCE, 1983, 261 (01) :45-48

[3]

BLUME A, 1993, PROG COLL POL SCI S, V93, P118

[4]

BLUME A, UNPUB

[5] SOLUBILIZATION OF LECITHIN VESICLES BY C12E8 - STRUCTURAL TRANSITIONS AND TEMPERATURE EFFECTS [J].

EDWARDS, K ;

ALMGREN, M .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 147 (01) :1-21

[6] ISOTHERMAL TITRATION CALORIMETRY [J].

FREIRE, E ;

MAYORGA, OL ;

STRAUME, M .

ANALYTICAL CHEMISTRY, 1990, 62 (18) :A950-A959

[7] MEMBRANE WATER PARTITION OF OLIGO(ETHYLENE OXIDE) DODECYL ETHERS AND ITS RELEVANCE FOR SOLUBILIZATION [J].

HEERKLOTZ, H ;

BINDER, H ;

LANTZSCH, G ;

KLOSE, G .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1994, 1196 (02) :114-122

[8]

HEERKLOTZ H, UNPUB

[9] SYSTEMATIC STUDY ON THE SOLUBILIZATION OF PHOSPHOLIPID-VESICLES BY VARIOUS SURFACTANTS [J].

INOUE, T ;

YAMAHATA, T ;

SHIMOZAWA, R .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1992, 149 (02) :345-358

[10]

Lantzsch G, 1994, J Fluoresc, V4, P339, DOI 10.1007/BF01881452

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