APPLICATION OF ISOTHERMAL TITRATION CALORIMETRY FOR DETECTING LIPID-MEMBRANE SOLUBILIZATION

被引：44

作者：

HEERKLOTZ, H ^{[1
]}

LANTZSCH, G ^{[1
]}

BINDER, H ^{[1
]}

KLOSE, G ^{[1
]}

BLUME, A ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,FACHBEREICH CHEM,D-67653 KAISERSLAUTERN,GERMANY

来源：

CHEMICAL PHYSICS LETTERS | 1995年 / 235卷 / 5-6期

关键词：

D O I：

10.1016/0009-2614(95)00139-U

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Isothermal titration calorimetry is presented to be an excellent method of detecting lipid membrane solubilization upon the addition of detergents. As an example, the titration of the non-ionic detergent C(12)EO(6) to the lipid POPC is studied. The main contribution to the measured titration heat is the detergent's enthalpy difference upon transfer between micelles and membranes. The detergent added as micelles is transferred into membranes up to the saturating detergent per lipid mol/mol ratio, R(sat) = 1.45 +/- 0.07. Beyond R(sat) the membranes are solubilized to micelles. Subsequently, the titration heat changes its sign from endothermic to exothermic. After complete membrane dissolution at R(sol) = 5.0 +/- 0.4 the titration heat vanishes nearly completely.

引用

页码：517 / 520

页数：4

共 15 条

[11] ADSORPTION OF CA2+ AND LA3+ TO BILAYER-MEMBRANES - MEASUREMENT OF THE ADSORPTION ENTHALPY AND BINDING CONSTANT WITH TITRATION CALORIMETRY [J].

LEHRMANN, R ;

SEELIG, J .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1994, 1189 (01) :89-95

[12] SOLUBILIZATION OF PHOSPHOLIPIDS BY DETERGENTS - STRUCTURAL AND KINETIC ASPECTS [J].

LICHTENBERG, D ;

ROBSON, RJ ;

DENNIS, EA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 737 (02) :285-304

[13] MICROTITRATION CALORIMETRIC STUDY OF THE MICELLIZATION OF 3 POLY(OXYETHYLENE) GLYCOL DODECYL ETHERS [J].

OLOFSSON, G .

JOURNAL OF PHYSICAL CHEMISTRY, 1985, 89 (08) :1473-1477

[14] MICELLE TO VESICLE TRANSITION IN AQUEOUS-SOLUTIONS OF BILE-SALT AND LECITHIN [J].

SCHURTENBERGER, P ;

MAZER, N ;

KANZIG, W .

JOURNAL OF PHYSICAL CHEMISTRY, 1985, 89 (06) :1042-1049

[15] RAPID MEASUREMENT OF BINDING CONSTANTS AND HEATS OF BINDING USING A NEW TITRATION CALORIMETER [J].

WISEMAN, T ;

WILLISTON, S ;

BRANDTS, JF ;

LIN, LN .

ANALYTICAL BIOCHEMISTRY, 1989, 179 (01) :131-137

← 1 2 →