CHARACTERIZATION OF [H-3] LSD BINDING TO A SEROTONIN-SENSITIVE SITE IN HONEYBEE (APIS-MELLIFERA) BRAIN

被引:17
作者
BLENAU, W [1 ]
MAY, T [1 ]
ERBER, J [1 ]
机构
[1] FREE UNIV BERLIN, INST NEUROPSYCHOPHARMAKOL, D-14050 BERLIN, GERMANY
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1995年 / 112卷 / 02期
关键词
5-HT RECEPTOR; H-3] LSD BINDING; HONEYBEE; INSECT BRAIN; SEROTONIN; NEUROMODULATOR;
D O I
10.1016/0305-0491(95)00098-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The binding of [H-3]lysergic acid diethylamide ([H-3]LSD) to a putative serotonin receptor site (S-site) in membrane preparations of honeybee brain was analyzed. All experiments were performed in the presence of 10 mu M dopamine to inhibit [H-3]LSD binding to an additional site, which is dopamine-sensitive (D-site), [H-3]LSD binds reversibly to the S-site with a K-D value of 0.89 nM. The maximal concentration of binding sites is 790 fmol/mg protein, Serotonin, lisuride, methysergide, 5-methoxytryptamine and tryptamine are the most potent displacers of S-site binding, The displacement efficacy of several other potential serotonin receptor agonists and antagonists was analyzed. Addition of the stable GTP analogue GTP gamma S or high sodium concentrations reduced specific [H-3]LSD binding to the putative serotonin receptor binding site.
引用
收藏
页码:377 / 384
页数:8
相关论文
共 43 条
[31]   LIGAND - A VERSATILE COMPUTERIZED APPROACH FOR CHARACTERIZATION OF LIGAND-BINDING SYSTEMS [J].
MUNSON, PJ ;
RODBARD, D .
ANALYTICAL BIOCHEMISTRY, 1980, 107 (01) :220-239
[32]   MAPPING AND ULTRASTRUCTURE OF SEROTONIN-IMMUNOREACTIVE NEURONS IN THE OPTIC LOBES OF 3 INSECT SPECIES [J].
NASSEL, DR ;
MEYER, EP ;
KLEMM, N .
JOURNAL OF COMPARATIVE NEUROLOGY, 1985, 232 (02) :190-204
[33]   SEROTONIN-IMMUNOREACTIVE NEURONS IN THE ANTENNAL LOBES AND SUBESOPHAGEAL GANGLION OF THE HONEYBEE [J].
REHDER, V ;
BICKER, G ;
HAMMER, M .
CELL AND TISSUE RESEARCH, 1987, 247 (01) :59-66
[34]  
RIENS H, 1992, 20TH P GOTT NEUR C, P525
[35]   BIOGENIC-AMINES AND THEIR RECEPTORS IN INSECTS [J].
ROEDER, T .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1994, 107 (01) :1-12
[36]   CLONING AND CHARACTERIZATION OF A DROSOPHILA TYRAMINE RECEPTOR [J].
SAUDOU, F ;
AMLAIKY, N ;
PLASSAT, JL ;
BORRELLI, E ;
HEN, R .
EMBO JOURNAL, 1990, 9 (11) :3611-3617
[37]   COMMON PROJECTION AREAS OF 5-HT-LIKE AND GABA-LIKE IMMUNOREACTIVE FIBERS IN THE VISUAL-SYSTEM OF THE HONEYBEE [J].
SCHAFER, S ;
BICKER, G .
BRAIN RESEARCH, 1986, 380 (02) :368-370
[38]   SEROTONIN-IMMUNOREACTIVE NEURONS IN THE BRAIN OF THE HONEYBEE [J].
SCHURMANN, FW ;
KLEMM, N .
JOURNAL OF COMPARATIVE NEUROLOGY, 1984, 225 (04) :570-580
[39]  
TAYLOR DJ, 1992, J COMP PHYSIOL A, V170, P715, DOI 10.1007/BF00198982
[40]   5-HYDROXYTRYPTAMINE IN INVERTEBRATES [J].
WALKER, RJ .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1984, 79 (02) :231-235