STUDIES ON PROTEINASES FROM CALOTROPIS-GIGANTEA LATEX .2. PHYSICOCHEMICAL PROPERTIES OF CALOTROPAIN-FI AND CALOTROPAIN-FII

被引：29

作者：

ABRAHAM, KI

JOSHI, PN

机构：

[1] Biochemistry Division, Department of Chemistry, University of Poona, Poona

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 568卷 / 01期

关键词：

Active site study; Calotropain; Milk clotting; Protease (Calotropis gigantea);

D O I：

10.1016/0005-2744(79)90279-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular weights of purified calotropain-FI and FII were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by gel filtration on Sephadex G-100. Activation of calotropain-FI and FII by different sulfhydryl activators was studied. The results obtained from inhibition studies by various enzyme-modifying reagents suggest the possible role of cysteine and histidine residues in the active site of both the enzymes. The free and total sulfhydryl contents of both the enzymes were determined by the use of 5-5′-dithio-bis-2-nitrobenzoic acid. Total amino acid compositions of both the enzymes were also determined. A comparative study of the esterase, amidase, milk-clotting and caseinolytic activities of calotropain-FI and FII are also presented. © 1979.

引用

页码：120 / 126

页数：7

共 29 条

[11] STUDIES ON FICIN .I. ITS ISOLATION AND CHARACTERIZATION [J].

ENGLUND, PT ;

KING, TP ;

CRAIG, LC ;

WALTI, A .

BIOCHEMISTRY, 1968, 7 (01) :163-&

[12] PURIFICATION OF YEAST PROTEINASES .I. FRACTIONATION AND SOME PROPERTIES OF PROTEINASES [J].

HATA, T ;

HAYASHI, R ;

DOI, E .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1967, 31 (02) :150-&

[13]

Jansen EF, 1941, J BIOL CHEM, V137, P459

[14]

KRAMER DE, 1964, J BIOL CHEM, V239, P2178

[15]

KRAMER DE, 1969, PLANT PHYSL, V44, P1566

[16]

KUNIMITSU DK, 1970, METHOD ENZYMOL, V19, P244

[17]

Liener I.E., 1970, METHOD ENZYMOL, V19, P261

[18] A STUDY OF NUMBER AND REACTIVITY OF SULFHYDRYL GROUPS OF FICIN [J].

LIENER, IE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1961, 53 (02) :332-&

[19]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[20]

MARINIBETTOLO GB, 1963, GAZZ CHIM ITAL, V93, P1239

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