LYSOZYME EXPRESSION IN LACTOCOCCUS-LACTIS

被引:22
作者
VANDEGUCHTE, M [1 ]
VANDERWAL, FJ [1 ]
KOK, J [1 ]
VENEMA, G [1 ]
机构
[1] UNIV GRONINGEN, CTR BIOL SCI, DEPT GENET, KERKLAAN 30, 9751 NN HAREN, NETHERLANDS
关键词
D O I
10.1007/BF00178174
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Three lysozyme-encoding genes, one of eukaryotic and two of prokaryotic origin, were expressed in Lactococcus lactis subsp. lactis. Hen egg white lysozyme (HEL) could be detected in L. lactis lysates by Western blotting. No lysozyme activity was observed, however, presumably because of the absence of correctly formed disulphide bonds in the L. lactis product. The functionally related lysozymes of the E. coli bacteriophages T4 and lambda were produced as biologically active proteins in L. lactis. In both cases, the highest expression levels were obtained using configurations in which the bacteriophage lysozyme genes had been translationally coupled to a short open reading frame of lactococcal origin. Both enzymes, like HEL, may prevent the growth of food-spoilage bacteria.
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页码:216 / 224
页数:9
相关论文
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