EXPRESSION OF MBP-HCV NS1/E2 FUSION PROTEIN IN ESCHERICHIA-COLI AND DETECTION OF ANTI-NS1/E2 ANTIBODY IN TYPE C CHRONIC LIVER-DISEASE

被引：22

作者：

MITA, E

HAYASHI, N

UEDA, K

KASAHARA, A

FUSAMOTO, H

TAKAMIZAWA, A

MATSUBARA, K

OKAYAMA, H

KAMADA, T

机构：

[1] OSAKA UNIV, RES FDN MICROBIAL DIS, KANNONJI, KAGAWA 768, JAPAN

[2] OSAKA UNIV, INST MOLEC & CELLULAR BIOL, SUITA, OSAKA 565, JAPAN

[3] OSAKA UNIV, MICROBIAL DIS RES INST, DEPT MOLEC GENET, SUITA, OSAKA 565, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80278-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize the putative NS1/E2 (non-structural protein 1/envelope 2) domain of HCV (hepatitis C virus), we expressed the hydrophilic three-quarters of this domain in a form of MBP (maltose binding protein) fusions in Escherichia coli. When we checked the positive frequency of antibody to this fusion protein, 17% of patients with type C chronic liver disease had this antibody. However, they were all positive for HCV-RNA in sera. These results suggest that the appearance of anti-NS1/E2 antibody does not serve as evidence of viral clearance. © 1992 Academic Press, Inc.

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页码：925 / 930

页数：6

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