EFFICIENT METHOD FOR THE SYNTHESIS AND PURIFICATION OF TRANS-[C-14]-GERANYLGERANYL PYROPHOSPHATE

被引：14

作者：

GAFNI, Y

SHECHTER, I

机构：

[1] Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel-Aviv University, Tel-Aviv

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90653-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure for the preparation and purification of trans-[14C]geranylgeranyl pyrophosphate from dl-[14C]mevalonate is described. This procedure involves biosynthesis of trans-[14C]geranylgeraniol from dl-[14C]mevalonate by cell-free extracts of the fungus Gibberella fujikuroi and chemical synthesis of trans-[14C]geranylgeranyl pyrophosphate from trans-[14C]geranylgeraniol. The purification of trans-[14C]geranylgeraniol is achieved by chromatography on a silver nitrate-impregnated silica gel column. The pyrophosphate ester is purified on a silica gel column. © 1979.

引用

页码：248 / 252

页数：5