ANALOG OF NEUROPEPTIDE-FF ATTENUATES MORPHINE ABSTINENCE SYNDROME

被引：52

作者：

MALIN, DH ^{[1
]}

LAKE, JR ^{[1
]}

LEYVA, JE ^{[1
]}

HAMMOND, MV ^{[1
]}

ROGILLIO, RB ^{[1
]}

ARCANGELI, KR ^{[1
]}

LUDGATE, K ^{[1
]}

MOORE, GM ^{[1
]}

PAYZA, K ^{[1
]}

机构：

[1] NICHHD, DEV NEUROBIOL LAB, BETHESDA, MD 20892 USA

来源：

PEPTIDES | 1991年 / 12卷 / 05期

关键词：

MORPHINE; OPIATE DEPENDENCE; OPIATE ABSTINENCE SYNDROME; F8FAMIDE; NEUROPEPTIDE-FF; MORPHINE-MODULATING PEPTIDE; FMRFAMIDE; ANTIOPIATE PEPTIDES;

D O I：

10.1016/0196-9781(91)90052-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The octapeptide FLFQPQRFamide (neuropeptide FF or F8Fa) may play a role in opiate dependence and subsequent abstinence syndrome. Previously, NPFF precipitated opiate abstinence syndrome, while IgG from NPFF antiserum attenuated subsequent naloxone-precipitated abstinence signs in dependent rats. The peptide desaminoYFLFQPQRamide (daY8Ra) was synthesized as a possible NPFF antagonist. At a dose of 600 ng ICV, daY8Ra significantly attenuated (p < 0.001) the number of abstinence-like signs subsequently induced by 10-mu-g NPFF ICV, suggesting that daY8Ra does have antagonist activity against NPFF. Pretreatment of morphine-dependent rats with the same dose of daY8Ra also significantly attenuated (p < 0.001) the abstinence signs subsequently precipitated by 10-mu-g naloxone ICV. Pretreatment with 600 ng of NPFF itself, or of NPFF modified at the N-terminal only (daY9Fa), failed to attenuate subsequent naloxone-precipitated abstinence, suggesting that the C-terminal modification is critical for NPFF antagonist activity. It should be noted, however, that higher doses of daY8Ra (2-mu-g or more) can precipitate some abstinence signs in a manner similar to NPFF.

引用

页码：1011 / 1014

页数：4

共 16 条

[1] CHARACTERIZATION OF RAT SPINAL-CORD RECEPTORS TO FLFQPQRFAMIDE, A MAMMALIAN MORPHINE MODULATING PEPTIDE - A BINDING STUDY [J].