ALTERNATIVELY-SPLICED P53 MESSENGER-RNA IN THE FAA-HTC1 RAT HEPATOMA-CELL LINE WITHOUT THE SPLICE SITE MUTATIONS

被引:12
作者
FUKUDA, I [1 ]
OGAWA, K [1 ]
机构
[1] ASAHIKAWA MED COLL, DEPT PATHOL, 4-5-3-11 NISHIKAGURA, ASAHIKAWA 078, JAPAN
关键词
P53; RT-PCR; EXON SKIPPING; FAA-HTC1; CELLS; HEPATOMA;
D O I
10.1247/csf.17.427
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A novel mutation of the p53 gene has been found in a rat hepatoma cell line, FAA-HTC1. This cell line carried two kinds of abnormal p53 transcripts; one lacked the exon 8 sequence, and the other had a single base substitution G to T which resulted in a new stop codon in exon 8. In the genomic DNA, this base substitution in exon 8 was present, indicating that both transcripts were transcribed from the mutated gene. No mutation was detected in its two flanking introns. In this cell line, the exon-deleted transcript seems to be generated by exon skipping due to an unknown mechanism other than splice site mutations.
引用
收藏
页码:427 / 432
页数:6
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