AGAR STANDARDS FOR QUANTITATIVE X-RAY-MICROANALYSIS OF RESIN-EMBEDDED PLANT-TISSUES

被引：26

作者：

FRITZ, E

JENTSCHKE, G

机构：

[1] Institute of Forest Botany, University of Göttingen, Göttingen, 37077

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 174卷

关键词：

AGAR STANDARD; FREEZE-DRYING; QUANTIFICATION; X-RAY MICROANALYSIS;

D O I：

10.1111/j.1365-2818.1994.tb04323.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Calibration standards for quantitative X-ray microanalysis of resin-embedded plant tissue were prepared by adding 6-600 mM KC1 to 5% agar. Agar blocks with an edge length of 1-2 mm were rapidly frozen, freeze-dried and embedded in styrene-methacrylate. Dry sections 1 mu m thick were mounted on adhesive-coated grids. Apart from fine-scale inhomogeneities caused by ice crystal formation, the KC1 is evenly distributed in the agar blocks. The peak-to-continuum values of K and Cl were highly linearly correlated to the K and Cl contents over the whole concentration range.

引用

页码：47 / 50

页数：4

共 20 条

[1]

BOYDE A, 1978, SCANNING ELECTRON MI, V178, P303

[2] INHOMOGENEITY IN EPOXY-RESIN STANDARDS FOR QUANTITATIVE BIOLOGICAL X-RAY-MICROANALYSIS [J].