DOUBLE-SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) WITH MURINE MONOCLONAL-ANTIBODIES FOR DETECTION OF STAPHYLOCOCCAL-ENTEROTOXIN-B

被引:2
作者
GOYACHE, J [1 ]
ORDEN, JA [1 ]
BLANCO, JL [1 ]
DOMENECH, A [1 ]
SUAREZ, G [1 ]
GOMEZLUCIA, E [1 ]
机构
[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT PATOL ANIM 1,E-28040 MADRID,SPAIN
来源
JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 1994年 / 41卷 / 10期
关键词
D O I
10.1111/j.1439-0450.1994.tb00274.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12(PO)) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts.
引用
收藏
页码:639 / 644
页数:6
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