ANALYSIS OF THE TRANSFER REGION OF THE STREPTOMYCES PLASMID SCP2-ASTERISK

被引：23

作者：

BROLLE, DF ^{[1
]}

PAPE, H ^{[1
]}

HOPWOOD, DA ^{[1
]}

KIESER, T ^{[1
]}

机构：

[1] UNIV MUNSTER, INST MIKROBIOL, W-4400 MUNSTER, GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 10卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb00912.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

pIJ903, a bifunctional derivative of the 31.4 kb low-copy-number, conjugative Streptomyces plasmid SCP2*, was mutagenized in Streptomyces lividans using Tn4560. Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties were isolated. The mutations defined five transfer-related genes, traA, traB, traC, traD and spd, clustered in a region of 9 kb. The deduced sequences of the putative TraA and TraB proteins showed no overall similarity to known protein sequences, but the phenotype of traA mutant plasmids and sequence motifs in the putative TraA protein suggested that it might be a DNA helicase.

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页码：157 / 170

页数：14

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