IN-VIVO REPRESSION BY A SITE-SPECIFIC DNA-BINDING PROTEIN DESIGNED AGAINST AN ONCOGENIC SEQUENCE

被引：238

作者：

CHOO, Y

SANCHEZGARCIA, I

KLUG, A

机构：

[1] Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, Hills Road

来源：

NATURE | 1994年 / 372卷 / 6507期

关键词：

D O I：

10.1038/372642a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A DNA-binding peptide comprising three zinc-fingers has been engineered to bind specifically to a unique nine-base-pair region of a BCR-ABL fusion oncogene in preference to the parent genomic sequences. Binding to the target oncogene in chromosomal DNA is possible In transformed cells in culture, and results in blockage of transcription. Consequently, murine cells rendered independent of growth factors by the action of the oncogene revert to factor dependence upon transient transfection with a vector expressing the peptide.

引用

页码：642 / 645

页数：4

共 37 条

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