COMPENSATION OF THE SCHLIEREN EFFECT IN FLOW-INJECTION ANALYSIS BY USING DUAL-WAVELENGTH SPECTROPHOTOMETRY

被引:99
作者
ZAGATTO, EAG
ARRUDA, MAZ
JACINTHO, AO
MATTOS, IL
机构
[1] Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, 13400 Piracicaba, SP
关键词
D O I
10.1016/S0003-2670(00)83550-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The Schlieren effect in flow-injection analysis is critically examined. Effects of sample constitution, injection volume, coil lengths, flow-rates, confluent stream addition and temperature are studied and practical implications in flow-injection design with and without the stopped-flow approach are discussed. For Schlieren compensation, a diode-array spectrophotometer is used and non-specific absorbance subtraction is exploited: the incident light passes through the flowing sample, the emergent light is dispersed, the intensities of two selected monochromatic beams are measured simultaneously by separate detectors and real-time subtraction of the wavelength-independent noise is achieved. The sensitivity and measurement precision improve after application of the subtraction algorithm. The potential, limitations and practical applications of the method are discussed. © 1990.
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页码:153 / 160
页数:8
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