ENDOSPORE HETEROGENEITY IN PASTEURIA-PENETRANS RELATED TO ADHESION TO PLANT-PARASITIC NEMATODES

被引：51

作者：

DAVIES, KG

REDDEN, M

PEARSON, TK

机构：

[1] Entomology and Nematology Department, Harpenden, Hertfordshire, Rothamsted Experimental Station

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 05期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00478.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridoma cell lines were screened by indirect immunofluorescence for the secretion of monoclonal antibodies (Mabs) to the surface of a population of endospores of the obligate nematode hyperparasite Pasteuria penetrans. Whereas polyclonal antibodies from test sera samples recognized 100% of the spores, five selected Mabs recognized different proportions of the spores ranging from 10 to 90% showing that the spore population was highly heterogeneous. Probing spores adhering to different nematode populations with the five Mabs showed that different subpopulations of the spores were specific to different nematode populations indicating cuticular heterogeneity among the nematode populations. Each of the five Mabs recognized a far larger proportion of the spores adhering to the nematode population on which the Pasteuria population was originally cultured than of those adhering to other populations.

引用

页码：370 / 373

页数：4

共 15 条

[1]

CHANNER A G D R, 1992, Fundamental and Applied Nematology, V15, P331

[2] INTERSPECIFIC DIFFERENCES IN THE NEMATODE SURFACE-COAT BETWEEN MELOIDOGYNE-INCOGNITA AND M-ARENARIA RELATED TO THE ADHESION OF THE BACTERIUM PASTEURIA-PENETRANS [J].