DERMATOFIBROSARCOMA PROTUBERANS - INCREASED GROWTH-RESPONSE TO PLATELET-DERIVED GROWTH FACTOR-BB IN CELL-CULTURE

被引：29

作者：

KIKUCHI, K ^{[1
]}

SOMA, Y ^{[1
]}

FUJIMOTO, M ^{[1
]}

KADONO, T ^{[1
]}

SATO, S ^{[1
]}

ABE, M ^{[1
]}

OHHARA, K ^{[1
]}

TAKEHARA, K ^{[1
]}

机构：

[1] TORANOMON GEN HOSP,DIV DERMATOL,MINATO KU,TOKYO 108,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2264

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dermatofibrosarcoma protuberans(DFSP) is a malignant tumor originating in the dermis. Although it is locally aggressive and recurs unless completely excised, it only rarely metastasizes. In the present study, we established 4 cultured DFSP cell strains, which were almost identical to normal skin fibroblasts when observed under a phase-contrast-microscope, and we observed their responses to various growth factors. DFSP cells showed significantly greater response to platelet-derived growth factor BB(PDGF BB) and transforming growth factor β1(TGFβ1) than normal fibroblasts. We also determined upregulation of PDGF β receptors in DFSP cells by both 125I PDGF-BB binding assay and immunoblotting analysis. These findings suggest that the interaction between the PDGF-B chain and the overexpression of PDGF β receptors might play a role in the development of DFSP tumors. © 1993 Academic Press, Inc.

引用

页码：409 / 415

页数：7

共 20 条

[1]

BOWENPOPE DF, 1982, J BIOL CHEM, V257, P5161

[2] NUCLEOTIDE-SEQUENCE OF THE SIMIAN SARCOMA-VIRUS GENOME - DEMONSTRATION THAT ITS ACQUIRED CELLULAR SEQUENCES ENCODE THE TRANSFORMING GENE-PRODUCT P28SIS [J].

DEVARE, SG ;

REDDY, EP ;

LAW, JD ;

ROBBINS, KC ;

AARONSON, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (03) :731-735

[3]

FRANKLIN WA, 1990, CANCER RES, V50, P6344

[4] BLOCKADE OF AUTOCRINE STIMULATION IN SIMIAN SARCOMA VIRUS-TRANSFORMED CELLS REVERSES DOWN-REGULATION OF PLATELET-DERIVED GROWTH-FACTOR RECEPTORS [J].

GARRETT, JS ;

COUGHLIN, SR ;

NIMAN, HL ;

TREMBLE, PM ;

GIELS, GM ;

WILLIAMS, LT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7466-7470

[5] ATTACHMENT OF SMOOTH-MUSCLE CELLS TO COLLAGEN AND THEIR MIGRATION TOWARD PLATELET-DERIVED GROWTH-FACTOR [J].

GROTENDORST, GR ;

SEPPA, HEJ ;

KLEINMAN, HK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3669-3672

[6] 2 CLASSES OF PDGF RECEPTOR RECOGNIZE DIFFERENT ISOFORMS OF PDGF [J].

HART, CE ;

FORSTROM, JW ;

KELLY, JD ;

SEIFERT, RA ;

SMITH, RA ;

ROSS, R ;

MURRAY, MJ ;

BOWENPOPE, DF .

SCIENCE, 1988, 240 (4858) :1529-1531

[7]

HELDIN CH, 1984, CELL, V37, P9, DOI 10.1016/0092-8674(84)90296-4

[8] BINDING OF DIFFERENT DIMERIC FORMS OF PDGF TO HUMAN-FIBROBLASTS - EVIDENCE FOR 2 SEPARATE RECEPTOR TYPES [J].

HELDIN, CH ;

BACKSTROM, G ;

OSTMAN, A ;

HAMMACHER, A ;

RONNSTRAND, L ;

RUBIN, K ;

NISTER, M ;

WESTERMARK, B .

EMBO JOURNAL, 1988, 7 (05) :1387-1393

[9]

HELDIN CH, 1990, CELL REGUL, V1, P556

[10] SURAMIN BINDS TO PLATELET-DERIVED GROWTH-FACTOR AND INHIBITS ITS BIOLOGICAL-ACTIVITY [J].

HOSANG, M .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1985, 29 (03) :265-273

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