TRANSFER OF PERSULFIDE SULFUR FROM THIOCYSTINE TO RHODANESE

被引：11

作者：

ABDOLRASULNIA, R ^{[1
]}

WOOD, JL ^{[1
]}

机构：

[1] UNIV TENNESSEE,CTR HLTH SCI,COLL BAS MED SCI,DEPT BIOCHEM,MEMPHIS,TN 38163

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 567卷 / 01期

关键词：

Persulfide sulfur; Rhodanese; Sulfur transferase; Thiocystine;

D O I：

10.1016/0005-2744(79)90180-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thiocystine (bis-[2-amino-2-carboxyethyl]trisulfide) is a natural substrate for rhodanese (thiosulfate;cyanide sulfurtransferase, EC 2.8.1.1). Analogs of thiocystine were prepared by eliminating the carboxyl or amino group or by lengthening the carbon chain. Of these only homothiocystine (bis-[2-amino-2-carboxypropyl]trisulfide) had appreciable activity as a substrate. At pH 8.6, the optimum for rhodanese, transfer of sulfane sulfur to cyanide in the presence of rhodanese was nonspecific. Only the sulfane sulfur of 35S-labeled thiocystine was transferred to rhodanese. Thus, thiocystine and thiosulfate both produce a rhodanese persulfide as a stable intermediate in sulfur transfer. © 1979.

引用

页码：135 / 143

页数：9

共 36 条

[1] RHODANESE-MEDIATED SULFUR TRANSFER TO SUCCINATE-DEHYDROGENASE [J].

BONOMI, F ;

PAGANI, S ;

CERLETTI, P ;

CANNELLA, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 72 (01) :17-24

[2]

BRANZOLI U, 1974, J BIOL CHEM, V249, P4346

[3] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].

BRAY, GA .

ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285

[4] ORGANIC DISULFIDES AND RELATED SUBSTANCES .20. A NOVEL PREPARATION OF SYMMETRICAL TRISULFIDES USING THIOLSULFONATES [J].

BUCKMAN, JD ;

FIELD, L .

JOURNAL OF ORGANIC CHEMISTRY, 1967, 32 (02) :454-&

[5] TITRATION OF RHODANESE WITH SUBSTRATES [J].

CANNELLA, C ;

PECCI, L ;

PENSA, B ;

COSTA, M ;

CAVALLINI, D .

FEBS LETTERS, 1974, 49 (01) :22-24

[6]

DAVIDSON B, 1965, J BIOL CHEM, V240, P4463

[7]

FIELD L, 1977, ORGANIC CHEM SULFUR, P331

[8] EFFECT OF SULFUR BINDING ON RHODANESE FLUORESCENCE [J].

FINAZZIAGRO, A ;

FEDERICI, G ;

CANNELLA, C ;

CAVALLINI, D ;

GIOVAGNOLI, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 28 (01) :89-+

[9]

FINAZZIAGRO A, 1971, FEBS LETT, V16, P172

[10] ACTIVATION OF PORCINE HEART MITOCHONDRIAL MALATE DEHYDROGENASE BY ZERO VALENCE SULFUR AND RHODANESE [J].

FINAZZIAGRO, A ;

MAVELLI, I ;

CANNELLA, C ;

FEDERICI, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 68 (02) :553-560

← 1 2 3 4 →