TRANSFER OF PERSULFIDE SULFUR FROM THIOCYSTINE TO RHODANESE

被引：11

作者：

ABDOLRASULNIA, R ^{[1
]}

WOOD, JL ^{[1
]}

机构：

[1] UNIV TENNESSEE,CTR HLTH SCI,COLL BAS MED SCI,DEPT BIOCHEM,MEMPHIS,TN 38163

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 567卷 / 01期

关键词：

Persulfide sulfur; Rhodanese; Sulfur transferase; Thiocystine;

D O I：

10.1016/0005-2744(79)90180-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thiocystine (bis-[2-amino-2-carboxyethyl]trisulfide) is a natural substrate for rhodanese (thiosulfate;cyanide sulfurtransferase, EC 2.8.1.1). Analogs of thiocystine were prepared by eliminating the carboxyl or amino group or by lengthening the carbon chain. Of these only homothiocystine (bis-[2-amino-2-carboxypropyl]trisulfide) had appreciable activity as a substrate. At pH 8.6, the optimum for rhodanese, transfer of sulfane sulfur to cyanide in the presence of rhodanese was nonspecific. Only the sulfane sulfur of 35S-labeled thiocystine was transferred to rhodanese. Thus, thiocystine and thiosulfate both produce a rhodanese persulfide as a stable intermediate in sulfur transfer. © 1979.

引用

页码：135 / 143

页数：9

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