SITE-SPECIFIC AMINO-TERMINAL MUTANTS OF YEAST-EXPRESSED BETA-ACTIN - CHARACTERIZATION OF THE INTERACTION WITH MYOSIN AND TROPOMYOSIN

被引:25
作者
ASPENSTROM, P
LINDBERG, U
KARLSSON, R
机构
[1] UNIV UPPSALA,CTR BIOMED,DEPT DEV BIOL,BOX 587,S-75123 UPPSALA,SWEDEN
[2] UNIV STOCKHOLM,WENNER GREN INST,DEPT ZOOL CELLBIOL,ARRHENIUS LABS E5,S-10691 STOCKHOLM,SWEDEN
关键词
YEAST; HETEROLOGOUS EXPRESSION; ACTIN MUTANT; MYOSIN INTERACTION;
D O I
10.1016/0014-5793(92)80477-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neutral or charge-shifting mutagenesis of beta-actin at positions 3 and 4 strongly influenced the actomyosin interaction under non-rigor conditions. The polymerization behaviour and tropomyosin binding properties on the other hand remained unaffected.
引用
收藏
页码:59 / 63
页数:5
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