MODIFICATION OF A CONVENTIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AUTOINJECTOR FOR USE WITH CAPILLARY LIQUID-CHROMATOGRAPHY

被引：12

作者：

SIMPSON, RC

机构：

[1] Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 691卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01053-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper will discuss the conversion of a conventional HPLC autoinjector to a format which is suitable for use with capillary HPLC. In addition to the actual modification procedure, the paper will also present data which characterize the performance of the modified injector. These data include items such as precision (approximately 1%), optimal valve loop loading volume (approximately 35-40 mu l), and absence of sample carryover between injections.

引用

页码：163 / 170

页数：8

共 5 条

[1]

CAPIELLO A, 1993, ANAL CHEM, V65, P1281

[2] ANALYSIS OF PEPTIDE MIXTURES BY CAPILLARY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A PRACTICAL GUIDE TO SMALL-SCALE SEPARATIONS [J].

DAVIS, MT ;

LEE, TD .

PROTEIN SCIENCE, 1992, 1 (07) :935-944

[3] SCREENING TECHNIQUES FOR THE DETECTION OF INBORN-ERRORS OF BILE-ACID METABOLISM BY DIRECT INJECTION AND MICRO-HIGH PERFORMANCE LIQUID-CHROMATOGRAPHY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY [J].

EVANS, JE ;

GHOSH, A ;

EVANS, BA ;

NATOWICZ, MR .

BIOLOGICAL MASS SPECTROMETRY, 1993, 22 (06) :331-337

[4] STRUCTURAL CHARACTERIZATION OF GLYCOPROTEIN DIGESTS BY MICROCOLUMN LIQUID-CHROMATOGRAPHY IONSPRAY TANDEM MASS-SPECTROMETRY [J].

LIU, JP ;

VOLK, KJ ;

KERNS, EH ;

KLOHR, SE ;

LEE, MS ;

ROSENBERG, IE .

JOURNAL OF CHROMATOGRAPHY, 1993, 632 (1-2) :45-56

[5] HIGH-SENSITIVITY ANALYSIS OF SIALYL-OLIGOSACCHARIDE GLYCOSYLATION SITES IN GLYCOPROTEINS BY MINIATURIZED TRYPTIC DIGESTION AND MICROCOLUMN LIQUID-CHROMATOGRAPHY [J].

SHIROTA, O ;

RICE, D ;

NOVOTNY, M .

ANALYTICAL BIOCHEMISTRY, 1992, 205 (02) :189-192

← 1 →