THE STABILITY OF OUTER-MEMBRANE PROTEIN AND ANTIGEN PROFILES OF A STRAIN OF BACTEROIDES-INTERMEDIUS GROWN IN CONTINUOUS CULTURE AT DIFFERENT PH AND GROWTH-RATES

The stability of the outer-membrane proteins and antigens of a strain of Bacteroides intermedius (VPI 8944 group genotype II) grown in contious culture at varying pH and growth rates (D = 0.025-0.2h-1, pH 6.0-7.3) has been measured. The membranes showed nine major proteins (> 67-19.55 kilodaltons) and six major antigens (65-28 kilodaltons). Membrane proteins and antigens were stable under the conditions tested; the major proteins were detected in all membranes, and the antigen profiles tested with different antisera showed maximum similarities of 82-95%. Differences did occur in the amounts of membrane proteins synthesized; cells at high growth rates and those growing on the surfaces in the chemostat showed increased amounts of two proteins (40 and 32 kilodaltons) and possibly novel proteins of 24 and 25 kilodaltons. In addition, these membranes reflected increased synthesis or a change to increased reactivity of antigens between 20.5 and 24 kilodaltons. The results indicate stability of the expression of outer-membrane proteins and antigens in environments of differing pH and under different growth rates. However, the amount of these molecules synthesized can vary, and increases in certain proteins and antigens occur as the growth rate increases and the organisms grow on surfaces.