THE NATURE OF EXTRACHROMOSOMAL MAINTENANCE OF TRANSFORMING PLASMIDS IN THE FILAMENTOUS BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM

被引：17

作者：

RANDALL, TA ^{[1
]}

REDDY, CA ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824

来源：

CURRENT GENETICS | 1992年 / 21卷 / 03期

关键词：

PHANEROCHAETE-CHRYSOSPORIUM; TRANSFORMATION; LIGNIN DEGRADATION; FILAMENTOUS FUNGI;

D O I：

10.1007/BF00336850

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.

引用

页码：255 / 260

页数：6

共 26 条

[1] TRANSFORMATION BY COMPLEMENTATION OF AN ADENINE AUXOTROPH OF THE LIGNIN-DEGRADING BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM [J].

ALIC, M ;

KORNEGAY, JR ;

PRIBNOW, D ;

GOLD, MH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (02) :406-411

[2] TRANSFORMATION OF PHANEROCHAETE-CHRYSOSPORIUM AND NEUROSPORA-CRASSA WITH ADENINE BIOSYNTHETIC GENES FROM SCHIZOPHYLLUM-COMMUNE [J].

ALIC, M ;

CLARK, EK ;

KORNEGAY, JR ;

GOLD, MH .

CURRENT GENETICS, 1990, 17 (04) :305-311

[3] ANALYSIS OF NUCLEOTIDE-SEQUENCES OF 2 LIGNINASE CDNAS FROM A WHITE-ROT FILAMENTOUS FUNGUS, PHANEROCHAETE-CHRYSOSPORIUM [J].

DEBOER, HA ;

ZHANG, YZ ;

COLLINS, C ;

REDDY, CA .

GENE, 1987, 60 (01) :93-102

[4] CHARACTERIZATION OF INL+ TRANSFORMANTS OF NEUROSPORA-CRASSA OBTAINED WITH A RECOMBINANT COSMID-POOL [J].

FEHER, Z ;

SCHABLIK, M ;

KISS, A ;

ZSINDELY, A ;

SZABO, G .

CURRENT GENETICS, 1986, 11 (02) :131-137

[5] TRANSFORMATION IN FUNGI [J].

FINCHAM, JRS .

MICROBIOLOGICAL REVIEWS, 1989, 53 (01) :148-170

[6] RECA-INDEPENDENT GENERAL GENETIC-RECOMBINATION OF PLASMIDS [J].

FISHEL, RA ;

JAMES, AA ;

KOLODNER, R .

NATURE, 1981, 294 (5837) :184-186

[7] DIFFERENTIAL PLASMID RESCUE FROM TRANSGENIC MOUSE DNAS INTO ESCHERICHIA-COLI METHYLATION-RESTRICTION MUTANTS [J].

GRANT, SGN ;

JESSEE, J ;

BLOOM, FR ;

HANAHAN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4645-4649

[8] GENETIC AND DNA-SEQUENCE ANALYSIS OF THE KANAMYCIN RESISTANCE TRANSPOSON TN903 [J].

GRINDLEY, NDF ;

JOYCE, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7176-7180

[9] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[10] ENZYMATIC COMBUSTION - THE MICROBIAL-DEGRADATION OF LIGNIN [J].

KIRK, TK ;

FARRELL, RL .

ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :465-505

← 1 2 3 →