THE NATURE OF EXTRACHROMOSOMAL MAINTENANCE OF TRANSFORMING PLASMIDS IN THE FILAMENTOUS BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM

被引：17

作者：

RANDALL, TA ^{[1
]}

REDDY, CA ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824

来源：

CURRENT GENETICS | 1992年 / 21卷 / 03期

关键词：

PHANEROCHAETE-CHRYSOSPORIUM; TRANSFORMATION; LIGNIN DEGRADATION; FILAMENTOUS FUNGI;

D O I：

10.1007/BF00336850

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.

引用

页码：255 / 260

页数：6

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