SCRAPE-LOADING - A SIMPLE METHOD TO INDUCE CHROMOSOMAL-ABERRATIONS WITH RESTRICTION ENZYMES IN CHO CELLS

被引：11

作者：

BOES, R ^{[1
]}

OBE, G ^{[1
]}

机构：

[1] UNIV GESAMTHSCH ESSEN,DEPT GENET,FACHBEREICH 9,POB 45037,D-45117 ESSEN,GERMANY

来源：

MUTATION RESEARCH | 1993年 / 292卷 / 03期

关键词：

SCRAPE-LOADING; RESTRICTION ENZYMES; CHROMOSOMAL ABERRATION INDUCTION;

D O I：

10.1016/0165-1161(93)90025-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The restriction endonucleases Alul and EcoRI induce chromosomal aberrations in Chinese hamster ovary (CHO) cells when applied via scrape-loading. The frequencies of chromosomal aberrations induced by AluI are similar to the ones induced when the enzyme is applied by other methods to introduce it into cells (electroporation, glycerol, sorbitol). EcoRI produces lower frequencies of aberrations when applied via scrape-loading, as compared to the application in the presence of high concentrations of glycerol, which may reflect relaxed activities under the latter conditions.

引用

页码：225 / 230

页数：6

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