A LIPID-COATED CATALYTIC ANTIBODY IN WATER-MISCIBLE ORGANIC-SOLVENTS

被引：20

作者：

OKAHATA, Y ^{[1
]}

YAMAGUCHI, M ^{[1
]}

TANAKA, F ^{[1
]}

FUJII, I ^{[1
]}

机构：

[1] PROT ENGN RES INST,SUITA,OSAKA 565,JAPAN

来源：

TETRAHEDRON | 1995年 / 51卷 / 28期

关键词：

D O I：

10.1016/0040-4020(95)00407-Y

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A lipid-coated catalytic antibody was prepared by mixing aqueous solutions of antibody and synthetic glycolipids. The lipid-coated catalytic antibody is soluble in organic solvents and showed a remarkable reactivity for hydrolysis of lipophilic esters in a buffer solution containing 20-80% DMSO (dimethyl sulfoxide). DMSO was added to solubilize the lipophilic esters and a native antibody was denatured in the same condition. Michaelis-Menten kinetics showed that the reduced reactivity of a native antibody in DMSO-buffer solutions is due to the largely deduced k(cat) value but not the small change of K-m, value.

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收藏

页码：7673 / 7680

页数：8

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