A SEMIEMPIRICAL APPROACH FOR THE SIMULATION OF CIRCULAR-DICHROISM SPECTRA OF GRAMICIDIN-A IN A MODEL MEMBRANE

被引：19

作者：

BANO, MC ^{[1
]}

BRACO, L ^{[1
]}

ABAD, C ^{[1
]}

机构：

[1] UNIV VALENCIA,DEPT BIOQUIM & BIOL MOLEC,E-46100 BURJASSOT,SPAIN

来源：

BIOPHYSICAL JOURNAL | 1992年 / 63卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(92)81590-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In an extension of our previous work (Bano, M. C., Braco, L., and Abad, C. 1991. Biochemistry. 30:886-94), the kinetics of dissociation of gramicidin A double-stranded dimers into beta-6.3-helical monomers in small unilamellar vesicles prepared following different protocols, were investigated using in combination circular dichroism (CD) and high-performance liquid chromatography (HPLC). The analysis of the data from both techniques according to a two-component model strongly supports that any given CD pattern of gramicidin incorporated in the phospholipid bilayer can be deconvoluted essentially as a linear combination of the reference subspectra calculated for the double-stranded dimer and the helical monomer. An HPLC-based, semi-empirical approach is proposed for the simulation of gramicidin CD curves in the model membrane used, and it is shown that the congruence between theoretical and experimental spectra is very satisfactory.

引用

页码：70 / 77

页数：8

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[31] SOLVENT DETERMINED CONFORMATION OF GRAMICIDIN AFFECTS THE ABILITY OF THE PEPTIDE TO INDUCE HEXAGONAL HII PHASE FORMATION IN DIOLEOYLPHOSPHATIDYLCHOLINE MODEL MEMBRANES [J].