HIGH-RESOLUTION 2-DIMENSIONAL MAPPING OF TISSUE-SPECIFIC POLYPEPTIDES IN THE DESERT LOCUST, SCHISTOCERCA-GREGARIA

被引：3

作者：

ANNADURAI, RS ^{[1
]}

OBERTHUR, W ^{[1
]}

REMBOLD, H ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,AM KLOPFERSPITZ 18A,W-8033 MARTINSRIED,GERMANY

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 07期

关键词：

D O I：

10.1002/elps.1150130195

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High resolution two-dimensional gel electrophoresis (2-DE) using immobilized pH gradients was used to map the tissue-specific polypeptides of the desert locust, Schistocerca gregaria. Highly specific comprehensive 2-DE reference maps ("master gels") were developed for the brain, corpus cardiacum, subesophageal ganglion, and hemolymph. The polypeptides were well resolved within the pH 4-7 range in the first dimension and within the 14-94 kDa molecular mass range in the second dimension.

引用

页码：456 / 458

页数：3

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