QUANTITATIVE-ANALYSIS OF 2-DIMENSIONAL PROTEIN PROFILES OF INBRED LINES OF MAIZE (ZEA-MAYS L)

被引：31

作者：

HIGGINBOTHAM, JW ^{[1
]}

SMITH, JSC ^{[1
]}

SMITH, OS ^{[1
]}

机构：

[1] PIONEER HI BRED INT INC, DIV PLANT BREEDING, DEPT DATA MANAGEMENT, JOHNSTON, IA USA

来源：

ELECTROPHORESIS | 1991年 / 12卷 / 06期

关键词：

D O I：

10.1002/elps.1150120608

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-dimensional electrophoresis and fluorography of [S-35]methionine labeled maize germinated embryo proteins were performed at Cold Spring Harbor Laboratory. Fluorographs of 63 gels representing 37 inbred lines were subsequently scanned and spot-detected at Protein and DNA Imageware Systems (Huntington Station, NY). The digitized images were then matched with the aid of PDQUEST-II computer software. Over 1500 different protein spots were included in the resulting dataset. The optical density data were normalized to parts per million, then transformed to their natural logarithms. Analyses of variance were performed on each spot in order to select for further study those spots with most of their variation partitioned among inbred lines rather than within inbred lines. Using this method of spot selection, over 100 protein spots were included in the set of spots which display significant differences among inbred lines of maize.

引用

页码：425 / 431

页数：7

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