ISOLATION OF A CDNA CLONE FOR BETA-1-4 GALACTOSYLTRANSFERASE FROM EMBRYONIC CHICKEN BRAIN AND COMPARISON TO ITS MAMMALIAN HOMOLOGS

被引：15

作者：

GHOSH, S ^{[1
]}

BASU, SS ^{[1
]}

BASU, S ^{[1
]}

机构：

[1] UNIV NOTRE DAME,DEPT CHEM & BIOCHEM,NOTRE DAME,IN 46556

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92334-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the observed biochemical and immunological similarities between bovine β1-4 galactosyltransferase (GalT) and a developmentally regulated galactosyltransferase (GalT-4; UDP-Gal:Lc3 β1-4 GalT) from embryonic chicken brain, a genetic similarity between the two enzymes has been postulated. To test our hypothesis, we have employed a reverse transcription-polymerase chain reaction (RT-PCR) based approach and isolated a ∼600 bp cDNA clone from embryonic chicken brain mRNA. Our results indicate that, within the ∼600 bp fragment, the avian cDNA nucleotide sequence is 74% homologous to the human β1-4 galactosyltransferase cDNA. Similarity and identity between the predicted amino acid sequences of the two galactosyltransferases are 75% and 61%, respectively. Similar to its mammalian counterparts, the embryonic chicken brain galactosyltransferase gene appears to encode multiple mRNA transcripts (2.3 and 1.4 kb) and shows multiple bands on a Southern blot (18.6,12.9,10.5 and 3.7 kb) indicating that the avian gene is either polyexonic and/or it belongs to a multiple gene family. © 1992.

引用

页码：1215 / 1222

页数：8

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