DOES PHOSPHOGLUCOISOMERASE DISPLAY ANOMERIC SPECIFICITY OR SELECTIVITY TOWARDS ALPHA-D-GLUCOSE 6-PHOSPHATE - AN ASSESSMENT BY 2-DIMENSIONAL PHASE-SENSITIVE P-31 EXCHANGE SPECTROSCOPY (EXSY) NMR

被引：5

作者：

KAYSER, F

BIESEMANS, M

WILLEM, R

MALAISSELAGAE, F

MALAISSE, WJ

机构：

[1] FREE UNIV BRUSSELS,ERASMUS MED SCH,EXPTL MED LAB,B-1070 BRUSSELS,BELGIUM

[2] FREE UNIV BRUSSELS,FAC ENGN,CTR HIGH RESOLUT NMR,B-1050 BRUSSELS,BELGIUM

[3] FREE UNIV BRUSSELS,FAC ENGN,DEPT GEN & ORGAN CHEM,B-1050 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL JOURNAL | 1993年 / 295卷

关键词：

D O I：

10.1042/bj2950607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study by two-dimensional phase-sensitive P-31 exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the enzyme displays anomeric selectivity rather than specificity, towards alpha-D-glucose 6-phosphate. Indeed, beta-D-glucose 6-phosphate participates for about 20% to the total and direct conversion of the aldohexose into oxohexose ester.

引用

页码：607 / 609

页数：3

共 16 条

[11] DETERMINATION OF THE KINETIC CONSTANTS OF GLUCOSEPHOSPHATE ISOMERASE BY NONLINEAR OPTIMIZATION - ISOMERIZATION AND ANOMERIZATION FUNCTION [J].