EXTRACELLULAR POLYSACCHARIDES FROM SUSPENSION-CULTURES OF NICOTIANA-PLUMBAGINIFOLIA

被引：100

作者：

SIMS, IM ^{[1
]}

BACIC, A ^{[1
]}

机构：

[1] UNIV MELBOURNE,SCH BOT,PLANT CELL BIOL RES CTR,SCH BOT,PARKVILLE,VIC 3052,AUSTRALIA

来源：

PHYTOCHEMISTRY | 1995年 / 38卷 / 06期

关键词：

NICOTIANA PLUMBAGINIFOLIA; SOLANACEAE; TOBACCO; CELL-SUSPENSION CULTURES; CARBOXYL REDUCTION; LINKAGE ANALYSIS; EXTRACELLULAR POLYSACCHARIDE;

D O I：

10.1016/0031-9422(94)00832-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction.

引用

页码：1397 / 1405

页数：9

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