HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NEOMYCIN IN MILK USING A HISEP COLUMN

被引：20

作者：

AGARWAL, VK

机构：

[1] The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 12期

关键词：

D O I：

10.1080/01483919008049047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high performance liquid chromatographic (HPLC) method for the determination of neomycin in milk is described. Milk is passed directly through an amberlite CG-50 ion exchange resin column, and the neomycin which is retained on the column is derivatized with ortho-phthalaldehyde (OPA) reagent. The derivatized neomycin is eluted from the column with potassium borate buffer/methanol and analyzed by HPLC. A HISEP HPLC column with fluorometric detection was used. Recoveries ranged from 94 to 102% in samples fortified between 0.1 to 5ppm levels. The detection limit is 50ppb. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：2475 / 2487

页数：13

共 11 条

[1] IMPROVED MICROBIOLOGICAL ASSAY PROCEDURES FOR DIHYDROSTREPTOMYCIN RESIDUES IN MILK AND DAIRY-PRODUCTS
INGLIS, JM
KATZ, SE
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 35 (03) : 517 - 520
[2] KATZ SE, 1978, J ASSOC OFF ANA CHEM, V61, P1103
[3] NELSON AA, 1951, FED PROC, V10, P366
[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR ANALYSES OF ANTIBIOTICS IN BIOLOGICAL-FLUIDS
NILSSONEHLE, I
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1983, 6 : 251 - 293
[5] REEVES DS, 1974, POSTGRAD MED J, V50, P20
[6] SHAIKH, IN PRESS J LIQUID CH
[7] COMPARATIVE STUDY OF RESPONSES TO NEOMYCINS B AND C BY MICROBIOLOGICAL AND GAS-LIQUID CHROMATOGRAPHIC ASSAY METHODS
TSUJI, K
ROBERTSON, JH
BAAS, R
MCINNIS, DJ
[J]. APPLIED MICROBIOLOGY, 1969, 18 (03) : 396 - +
[8] A CLINICAL APPRAISAL OF NEOMYCIN
WAISBREN, BA
SPINK, WW
[J]. ANNALS OF INTERNAL MEDICINE, 1950, 33 (05) : 1099 - 1119
[9] WAKSMAN SA, 1950, J LAB CLIN MED, V36, P93
[10] 1974, MICROBIOLOGY LABOR 6, P315

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