METHOD TO IDENTIFY SPECIFIC ALLELES OF A PHANEROCHAETE-CHRYSOSPORIUM GENE ENCODING LIGNIN PEROXIDASE

被引：12

作者：

GASKELL, J

VANDENWYMELENBERG, A

STEWART, P

CULLEN, D

机构：

[1] US FOREST SERV,FOREST PROD LAB,INST MICROBIAL & BIOCHEM TECHNOL,1 GIFFORD PINCHOT DR,MADISON,WI 53705

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 04期

关键词：

D O I：

10.1128/AEM.58.4.1379-1381.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.

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页码：1379 / 1381

页数：3

相关论文

共 21 条

[1] MATING SYSTEM AND BASIDIOSPORE FORMATION IN THE LIGNIN-DEGRADING BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM [J].

ALIC, M ;

LETZRING, C ;

GOLD, MH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (07) :1464-1469

[2] CHARACTERIZATION OF 2 LIGNIN PEROXIDASE CLONES FROM PHANEROCHAETE-CHRYSOSPORIUM [J].

ANDRAWIS, A ;

PEASE, EA ;

KUAN, IC ;

HOLZBAUR, E ;

TIEN, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (02) :673-680

[3] CLONING AND SEQUENCING OF A LIGNINASE GENE FROM A LIGNIN-DEGRADING BASIDIOMYCETE, PHANEROCHAETE-CHRYSOSPORIUM [J].

ASADA, Y ;

KIMURA, Y ;

KUWAHARA, M ;

TSUKAMOTO, A ;

KOIDE, K ;

OKA, A ;

TAKANAMI, M .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 29 (05) :469-473

[4]

BLANCHETTE RA, IN PRESS EVALUATING

[5] MULTIPLE LIGNINASE-RELATED GENES FROM PHANEROCHAETE-CHRYSOSPORIUM [J].

BROWN, A ;

SIMS, PFG ;

RAEDER, U ;

BRODA, P .

GENE, 1988, 73 (01) :77-85

[6] ANALYSIS OF NUCLEOTIDE-SEQUENCES OF 2 LIGNINASE CDNAS FROM A WHITE-ROT FILAMENTOUS FUNGUS, PHANEROCHAETE-CHRYSOSPORIUM [J].

DEBOER, HA ;

ZHANG, YZ ;

COLLINS, C ;

REDDY, CA .

GENE, 1987, 60 (01) :93-102

[7] GENOMIC ORGANIZATION OF LIGNIN PEROXIDASE GENES OF PHANEROCHAETE-CHRYSOSPORIUM [J].

GASKELL, J ;

DIEPERINK, E ;

CULLEN, D .

NUCLEIC ACIDS RESEARCH, 1991, 19 (03) :599-603

[8] CONDITIONS FOR FRUIT BODY FORMATION IN THE WHITE ROT BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM [J].

GOLD, MH ;

CHENG, TM .

ARCHIVES OF MICROBIOLOGY, 1979, 121 (01) :37-41

[9] CHARACTERIZATION OF LIGNIN PEROXIDASE-ENCODING GENES FROM LIGNIN-DEGRADING BASIDIOMYCETES [J].

HUOPONEN, K ;

OLLIKKA, P ;

KALIN, M ;

WALTHER, I ;

MANTSALA, P ;

REISER, J .

GENE, 1990, 89 (01) :145-150

[10] ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA AND DOUBLE-STRANDED RNAS FROM FUNGI [J].

KIM, WK ;

MAUTHE, W ;

HAUSNER, G ;

KLASSEN, GR .

CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1990, 68 (09) :1898-1902