By interspecific hybridization within the genus Brassica, trigenomic haploids were produced and backcrossed four times with B. napus, variety 'Andor'. From this material, monosomic B-genome chromosome addition lines were selected with the extra chromosome derived from three different B-genome sources, i.e., B. nigra (BB), B. carinata (BBCC), and B. juncea (AABB). After selfing and/or microspore culture, disomic addition lines were obtained. Meiotic behavior was studied of the trigenomic hybrids, the pentaploid BC1 plants, and the monosomic addition lines. The addition lines were shown to possess cytological stability and good fertility.