EFFECTS OF RADIATION-DAMAGE WITH 400-KV ELECTRONS ON FROZEN, HYDRATED ACTIN BUNDLES

被引：16

作者：

SCHMID, MF

JAKANA, J

MATSUDAIRA, P

CHIU, W

机构：

[1] BAYLOR COLL MED, VERNA & MARRS MCLEAN DEPT BIOCHEM, HOUSTON, TX 77030 USA

[2] MIT, WHITEHEAD INST BIOMED RES, CAMBRIDGE, MA 02142 USA

[3] MIT, DEPT BIOL, CAMBRIDGE, MA 02142 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1992年 / 108卷 / 01期

关键词：

D O I：

10.1016/1047-8477(92)90007-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron images can be used to provide amplitudes and phases for the structural determination of biological specimens. Radiation damage limits the amount of structural information retrievable by computer processing. A 400-kV electron microscope was used to investigate radiation damage effects on frozen, hydrated actin bundles kept at -168°C. The quality of phases within and among images in a damage series was evaluated quantitatively out to 16 Å resolution. It was found that the phases of structure factors with good signal-to-noise ratio (IQ \ ̌ 4) can be reliably retrieved from images taken at a cumulative dose of at least 25 electrons/Å2. © 1992.

引用

页码：62 / 68

页数：7

共 13 条

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