A 4TH CLASS OF THETA-REPLICATING PLASMIDS - THE PAM-BETA-1 FAMILY FROM GRAM-POSITIVE BACTERIA

被引：95

作者：

BRUAND, C

LECHATELIER, E

EHRLICH, SD

JANNIERE, L

机构：

[1] Laboratoire de Genetique Microbienne, INRA, 78352 Jouy en Josas Cedex, Domaine de Vilvert

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 24期

关键词：

REPLICATION PROTEIN; REPLICATION ORIGIN; DNA POLYMERASE-I; SNAP-BACK REPLICATION; PROMISCUOUS REPLICATION;

D O I：

10.1073/pnas.90.24.11668

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmid pAMbeta1 from Enterococcus faecalis uses a unidirectional theta mode of replication. We show here that this replication (i) is dependent on a plasmid-encoded replication protein (Rep) but not on a DNA structure typical for origins of most Rep-dependent plasmids and (ii) is initiated by DNA polymerase I (PolI). pAMbeta1 minimal replicon shares no homology with highly conserved ColE1-type replicons, which use Poll for initiation but do not encode a Rep, or with ColE2 and ColE3 replicons, which require PolI for replication and encode a Rep. We propose that pAMbeta1 and a number of other naturally occurring and closely related plasmids form a distinct plasmid class.

引用

页码：11668 / 11672

页数：5

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