MOLECULAR-CLONING OF CDNAS ENCODING HUMAN GLEPP1, A MEMBRANE-PROTEIN TYROSINE PHOSPHATASE - CHARACTERIZATION OF THE GLEPP1 PROTEIN DISTRIBUTION IN HUMAN KIDNEY AND ASSIGNMENT OF THE GLEPP1 GENE TO HUMAN-CHROMOSOME 12P12-P13

Human glomerular epithelial protein 1 (GLEPP1), a receptor-like membrane protein tyrosine phosphatase (PTPase), was cloned and sequenced from a human renal cortical cDNA library, The human nucleotide and derived amino acid sequences were, respectively, 90 and 97% identical to those of rabbit, Human GLEPP1 is predicted to contain 1188 amino acids, The predicted mature protein is 1159 amino acids long and contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Monoclonal and polyclonal antibodies raised against a human GLEPP1 fusion protein recognized a protein with distribution restricted to the glomerulus in human kidney and with an apparent molecular weight of approximately 200 kDa, The GLEPP1 gene was assigned to human chromosome 12p12-p13 by fluorescence in situ hybridization. (C) 1995 Academic Press, Inc.