ORIGIN OF VARIABILITY IN QUANTAL SIZE IN CULTURED HIPPOCAMPAL-NEURONS AND HIPPOCAMPAL SLICES

被引：377

作者：

BEKKERS, JM ^{[1
]}

RICHERSON, GB ^{[1
]}

STEVENS, CF ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT NEUROL,NEW HAVEN,CT 06510

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 14期

关键词：

Brain slice; Miniature synaptic currents; Patch clamp;

D O I：

10.1073/pnas.87.14.5359

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The size of synaptic quanta has been found to display considerable variation in cultured hippocampal neurons, but the source of this variability was previously unknown. We have now compared the properties of locally evoked miniature excitatory postsynaptic currents in cultured hippocampal neurons and in thin hippocampal slices using whole-cell patch-clamp recordings. The variability in miniature excitatory postsynaptic current size was similar in both preparations and occurred in cultured neurons when only one or a few synaptic boutons were stimulated. Thus, the variability in miniature excitatory postsynaptic current amplitude is not an artifact of cultured neurons and arises predominantly from variability within a single bouton. Possible origins of this variability are discussed.

引用

页码：5359 / 5362

页数：4