THE COMPLETE AMINO-ACID-SEQUENCE OF A THROMBIN-LIKE ENZYME GYROXIN ANALOG FROM VENOM OF THE BUSHMASTER SNAKE (LACHESIS-MUTA-MUTA)

被引：46

作者：

MAGALHAES, A

DAFONSECA, BCB

DINIZ, CR

GILROY, J

RICHARDSON, M

机构：

[1] UNIV DURHAM,DEPT BIOL SCI,SCI LABS,SOUTH RD,DURHAM DH1 3LE,ENGLAND

[2] FUNDACAO EZEQUIEL DIAS,CTR PESQUISA & DESENVOLVIMENTO,BR-30510-010 BELO HORIZON,MG,BRAZIL

来源：

FEBS LETTERS | 1993年 / 329卷 / 1-2期

关键词：

SNAKE VENOM; AMINO ACID SEQUENCE; THROMBIN-LIKE ENZYME; GYROXIN ANALOG; LACHESIS-MUTA-MUTA;

D O I：

10.1016/0014-5793(93)80205-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of a thrombin-like enzyme with gyroxin activity isolated from the venom of the bushmaster snake Lachesis muta muta was determined by automated and DABITC/PITC microsequencing of the intact protein; fragments derived from it by separate cleavages with cyanogen bromide, iodosobenzoic acid and hydroxylamine; and peptides resulting from enzymatic digestions with trypsin, pepsin, chymotrypsin, and elastase. The protein, which is composed of 228 residues, contains four putative sites of N-linked glycosylation and exhibits significant sequence similarities with other serine proteases reported from snake venoms.

引用

页码：116 / 120

页数：5

共 30 条

[21] CATROXOBIN, A WEAKLY THROMBIN-LIKE ENZYME FROM THE VENOM OF CROTALUS-ATROX - NH2-TERMINAL AND ACTIVE-SITE AMINO-ACID SEQUENCES [J].