THERMAL-STABILITY OF THE POLYHEME CYTOCHROME C(3) SUPERFAMILY

被引：14

作者：

FLORENS, L

BIANCO, P

HALADJIAN, J

BRUSCHI, M

PROTASEVICH, I

MAKAROV, A

机构：

[1] UNIV AIX MARSEILLE 1, CNRS, CHIM & ELECTROCHIM COMPLEXES LAB, F-13331 MARSEILLE 3, FRANCE

[2] RUSSIAN ACAD SCI, ENGELHARDT INST MOLEC BIOL, MOSCOW 117984, RUSSIA

来源：

FEBS LETTERS | 1995年 / 373卷 / 03期

关键词：

POLYHEME CYTOCHROMES C; THERMAL STABILITY; STRUCTURE FUNCTION RELATIONSHIPS;

D O I：

10.1016/0014-5793(95)01062-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytochrome c(3) superfamily includes Desulfovibrio polyheme cytochromes c. We report the characteristic thermal stability parameters of the Desalfovibrio desulfuricans Norway (D.d.N.) cytochromes c(3) (M(r) 13,000 and M(r) 26,000) and the Desulfovibrio vulgaris Hildenborough (D.v.H.) cytochrome c(3) (M(r) 13,000) and high molecular mass cytochrome c (Hmc), as obtained with the help of electronic spectroscopy, voltammetric techniques and differential scanning calorimetry. The polyheme cytochromes are denatured over a wide range of temperatures: the D.v.H. cytochrome c(3) is highly thermostable (T-d = 121 degrees C) contrary to the D.d.N. protein (T-d = 73 degrees C), The thermostability of the polyheme cytochromes is redox state dependent. The results are discussed in the light of the structural and functional relationships within the cytochrome c(3) superfamily.

引用

收藏

页码：280 / 284

页数：5

相关论文

共 33 条

[1] AMINO ACID SEQUENCE OF CYTOCHROME C-551.5 (CYTOCHROME C7) FROM GREEN PHOTOSYNTHETIC BACTERIUM CHLOROPSEUDOMONAS-ETHYLICA [J].

AMBLER, RP .

FEBS LETTERS, 1971, 18 (02) :351-&

[2] SEQUENCE VARIABILITY IN BACTERIAL CYTOCHROMES-C [J].

AMBLER, RP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1058 (01) :42-47

[3] CORRELATIONS STUDIES BETWEEN STRUCTURAL AND REDOX PROPERTIES OF CYTOCHROMES-C3 [J].

BRUSCHI, M ;

LOUTFI, M ;

BIANCO, P ;

HALADJIAN, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (02) :384-389

[4] PURIFICATION AND CHARACTERIZATION OF CYTOCHROME C-3, FERREDOXIN, AND RUBREDOXIN ISOLATED FROM DESULFOVIBRIO-DESULFURICANS NORWAY [J].

BRUSCHI, M ;

HATCHIKIAN, CE ;

GOLOVLEVA, LA ;

LEGALL, J .

JOURNAL OF BACTERIOLOGY, 1977, 129 (01) :30-38

[5] BIOCHEMICAL AND SPECTROSCOPIC CHARACTERIZATION OF THE HIGH-MOLECULAR-WEIGHT CYTOCHROME-C FROM DESULFOVIBRIO-VULGARIS HILDENBOROUGH EXPRESSED IN DESULFOVIBRIO-DESULFURICANS G200 [J].

BRUSCHI, M ;

BERTRAND, P ;

MORE, C ;

LEROY, G ;

BONICEL, J ;

HALADJIAN, J ;

CHOTTARD, G ;

POLLOCK, WBR ;

VOORDOUW, G .

BIOCHEMISTRY, 1992, 31 (12) :3281-3288

[6] CRYSTAL-STRUCTURE OF CYTOCHROME C(3) FROM DESULFOVIBRIO-DESULFURICANS NORWAY AT 1-CENTER-DOT-7 ANGSTROM RESOLUTION [J].

CZJZEK, M ;

PAYAN, F ;

GUERLESQUIN, F ;

BRUSCHI, M ;

HASER, R .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (04) :653-667

[7] MOLECULAR AND STRUCTURAL BASIS OF ELECTRON-TRANSFER IN TETRA-HEME AND OCTA-HEME CYTOCHROMES [J].

CZJZEK, M ;

PAYAN, F ;

HASER, R .

BIOCHIMIE, 1994, 76 (06) :546-553

[8] DRASTIC INFLUENCE OF A SINGLE HEME AXIAL LIGAND REPLACEMENT ON THE THERMOSTABILITY OF CYTOCHROME C(3) [J].

DOLLA, A ;

FLORENS, L ;

BRUSCHI, M ;

DUDICH, IV ;

MAKAROV, AA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 211 (03) :742-747

[9] RECENT ADVANCES IN THE CHARACTERIZATION OF THE HEXADECAHEMIC CYTOCHROME-C FROM DESULFOVIBRIO [J].

FLORENS, L ;

BRUSCHI, M .

BIOCHIMIE, 1994, 76 (06) :561-568

[10] ON THE ORIGIN OF THE ENTHALPY AND ENTROPY CONVERGENCE TEMPERATURES IN PROTEIN FOLDING [J].

FU, L ;

FREIRE, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9335-9338

← 1 2 3 4 →