CLONING OF A NOVEL BACTERIA-BINDING RECEPTOR STRUCTURALLY RELATED TO SCAVENGER RECEPTORS AND EXPRESSED IN A SUBSET OF MACROPHAGES

被引:409
作者
ELOMAA, O
KANGAS, M
SAHLBERG, C
TUUKKANEN, J
SORMUNEN, R
LIAKKA, A
THESLEFF, I
KRAAL, G
TRYGGVASON, K
机构
[1] UNIV OULU, BIOCTR, SF-90570 OULU, FINLAND
[2] UNIV OULU, DEPT BIOCHEM, SF-90570 OULU, FINLAND
[3] UNIV HELSINKI, DEPT PEDODONT & ORTHODONT, SF-00014 HELSINKI, FINLAND
[4] UNIV OULU, BIOCTR, SF-90220 OULU, FINLAND
[5] UNIV OULU, DEPT ANAT, SF-90570 OULU, FINLAND
[6] UNIV OULU, DEPT PATHOL, SF-90570 OULU, FINLAND
[7] FREE UNIV AMSTERDAM, DEPT CELL BIOL, 1085 BT AMSTERDAM, NETHERLANDS
基金
芬兰科学院;
关键词
D O I
10.1016/0092-8674(95)90514-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel murine plasma membrane protein has been identified in subpopulations of macrophages. It has an intracellular N-terminal domain, a transmembrane domain, and an extracellular region with a short spacer, an 89 Gly-Xaa-Yaa repeat-containing collagenous domain, and a C-terminal cysteine-rich domain. In situ hybridization and immunohistochemical staining have localized the protein to a subset of macrophages in the marginal zone of the spleen and the medullary cord of lymph nodes. No expression was observed in macrophages of liver or lung. Transfected COS cells synthesized a native trimeric plasma membrane protein that bound labeled bacteria and acetylated LDL, but not yeast or Ficoll. The results suggest that the novel protein is a macrophage-specific membrane receptor with a role in host defense, as it shows postnatal expression in macrophages, which are considered responsible far the binding of bacterial antigens and phagocytosis.
引用
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页码:603 / 609
页数:7
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