ISOLATION OF IGG FROM MAMMALIAN SERA WITH AID OF CAPRYLIC ACID

被引:900
作者
STEINBUCH, M
AUDRAN, R
机构
[1] Laboratory of Fractionation and Biochemistry, Centre National de Transfusion Sanguine, Paris
关键词
D O I
10.1016/0003-9861(69)90285-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A technique is described for the precipitation of the bulk of the plasma proteins with caprylic (octanoic) acid without affecting IgG, ceruloplasmin, and part of the IgA. The contaminants can be removed from the supernatant solution by batch-adsorption on DEAE-cellulose and pure IgG is obtained in good yield. The technique has been used for human, horse, sheep, and rabbit plasma. © 1969.
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页码:279 / +
页数:1
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