ISOLATION OF XYLOSE REDUCTASE GENE OF PICHIA-STIPITIS AND ITS EXPRESSION IN SACCHAROMYCES-CEREVISIAE

被引：54

作者：

TAKUMA, S ^{[1
]}

NAKASHIMA, N ^{[1
]}

TANTIRUNGKIJ, M ^{[1
]}

KINOSHITA, S ^{[1
]}

OKADA, H ^{[1
]}

SEKI, T ^{[1
]}

YOSHIDA, T ^{[1
]}

机构：

[1] OSAKA UNIV,FAC ENGN,INT CTR COOPERAT RES BIOTECHNOL,2-1 YAMADA OKA,SUITA,OSAKA 565,JAPAN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 28-9卷

关键词：

XYLOSE REDUCTASE; DNA SEQUENCE; GENE EXPRESSION; PICHIA-STIPITIS; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1007/BF02922612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation in P stipitis were inducible. S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol by S. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.

引用

页码：327 / 340

页数：14

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