ENZYME-IMMOBILIZED SH-SAW BIOSENSOR

被引：21

作者：

KONDOH, J

MATSUI, Y

SHIOKAWA, S

WLODARSKI, WB

机构：

[1] SHIZUOKA UNIV,GRAD SCH ELECTR SCI & TECHNOL,HAMAMATSU,SHIZUOKA 432,JAPAN

[2] SHIZUOKA UNIV,FAC ENGN,HAMAMATSU,SHIZUOKA 432,JAPAN

[3] ROYAL MELBOURNE INST TECHNOL,MELBOURNE,VIC 3000,AUSTRALIA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1994年 / 20卷 / 2-3期

关键词：

D O I：

10.1016/0925-4005(94)01182-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A shear horizontal mode surface acoustic wave (SH-SAW) device has many attractions for chemical-sensing applications in liquid media. The SH-SAW that propagates on 36 degrees rotated Y-cut, X-propagating LiTaO3 is affected by a strong acoustoelectric interaction between the piezoelectric potential and electric properties of the adjacent liquid. This paper describes an enzyme-immobilized SH-SAW biosensor in which the detection principle is the acoustoelectric interaction due to the pH change in the enzyme reaction. Two kinds of enzymes, urease and cholinesterase, are immobilized on the sensor surface and highly sensitive detection of the enzyme reaction is obtained experimentally. The urease-immobilized SH-SAW biosensor is implemented in a liquid-flow system. By using the cholinesterase-immobilized SH-SAW sensor, we have succeeded in detecting an inhibition effect of a pesticide.

引用

页码：199 / 203

页数：5

共 10 条

[1] PROPAGATION OF SURFACE WAVES AT BOUNDARY BETWEEN A PIEZOELECTRIC CRYSTAL AND A FLUID MEDIUM [J].