HUMAN THYMOCYTE ANTIGEN DEFINED BY A HYBRID MYELOMA MONOCLONAL ANTIBODY

被引：527

作者：

MCMICHAEL, AJ

PILCH, JR

GALFRE, G

MASON, DY

FABRE, JW

MILSTEIN, C

机构：

[1] UNIV OXFORD, RADCLIFFE INFIRM, DEPT PATHOL, OXFORD OX2 6HE, ENGLAND

[2] MRC, MOLEC BIOL LAB, CAMBRIDGE, ENGLAND

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1979年 / 9卷 / 03期

关键词：

D O I：

10.1002/eji.1830090307

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Spleen cells from a BALB/c mouse that immunized with human thymocytes and fused with the myeloma line P3-NS1/1 Ag 4.1. One of the resulting hybrid clones (NA1/34) secreted an antibody that was highly specific for human thymocytes. Of the thymocytes 85% expressed the antigen designated HTA1. There were an estimated 15 .times. 104 molecules of HTA1/cell, and it was therefore, a major surface molecule. The expression of this antigen on thymocytes appeared reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 MW.

引用

页码：205 / 210

页数：6