FED-BATCH CULTURE AUTOMATED BY USES OF CONTINUOUSLY MEASURED CELL CONCENTRATION AND CULTURE VOLUME

被引：38

作者：

YAMANE, T ^{[1
]}

HIBINO, W ^{[1
]}

ISHIHARA, K ^{[1
]}

KADOTANI, Y ^{[1
]}

KOMINAMI, M ^{[1
]}

机构：

[1] CENT ENGN & CONSTRUCT CO LTD,NAKAMURA KU,NAGOYA 450,JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 05期

关键词：

FED-BATCH CULTURE; AUTOMATION OF FEEDING; LASER TURBIDIMETER;

D O I：

10.1002/bit.260390510

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An automated control method of fed-batch culture in which the nutrient feed rate was determined from continuously measured cell concentration and culture broth volume was developed. Theoretical background was elucidated, from which it was found that the method is unique in that it controls specific substrate consumption rate of the microorganism. The method was experimentally applied to the fed-batch cultures of recombinant Escherichia coli HB101. It was observed that the specific substrate feed rate affects not only the specific growth rate but also the growth yield. If some conditions are satisfied, this type of automated fed-batch culture can be applied widely to any microbial systems and seems especially useful when the culture medium is composed of natural complex nutrient(s) because their concentrations are very difficult to detect and control.

引用

页码：550 / 555

页数：6